miR-720 Regulates Insulin Secretion by Targeting Rab35

miRNAs pose a good prospect in the diagnosis and treatment of type 2 diabetes (T2D). This study is aimed at investigating whether miR-720 targets Rab35 to regulate insulin secretion MIN6 cells its molecular mechanism clinical value as specific biomarker T2D. Fifty-five samples new T2D patients normal control were collected. Levels miR-720, fasting blood glucose, insulin, other indicators glucose lipid metabolism determined. We increased decreased expression using mimic inhibitor identify effect on cells, respectively. Then, we used mimic, inhibitor, dual luciferase reporter gene assays prove which regulates by targeting cells. In addition, overexpressed silenced detect PI3K, Akt, mTOR RT-PCR western blot. this study, circulating was significantly higher group than group, miR-270 positive correlated with FBG, while negatively FINS. The overexpression inhibited secretion, downregulation promoted secretion. regulated Compared gene, silencing could induce mTOR. Furthermore, activate PI3K pathway. conclude that may be potential for Increase reduced then PI3K/Akt/mTOR signal pathway, thus inhibiting